![]() ![]() Spores are structures remarkably resistant to heat, radiation, chemicals and other agents that are typically lethal to the organism. Principle: Endospore staining is a differential staining technique where the spore is stained in a manner so that it can be distinguished from the vegetative part of the cell. What is the principle of Endospore staining? In this technique heating acts as a mordant. Because endospores resist staining, the malachite green will be forced into (i.e, malachite green permeate the spore wall) the endospores by heating. ![]() However, once stained, endospores are quite resistant to decolorization.Īlso, what stains are used in an Endospore stain?Ī primary stain (malachite green) is used to stain the endospores. ![]() Endospores strongly resist application of simple stains or dyes and hence appear as nonstaining entities in Gram- stain preparations. Spores are resistant to heat, dessication, chemicals, and radiation.Īlso, what do endospores look like in a Gram stain? When viewed unstained, endospores of living bacilli appear edged in black and are very bright and refractile. By forming spores, bacteria can survive in hostile conditions. are two examples of endospore-forming bacteria. Its primary application is to locate and recognize the existence of bacterial endospores as well as vegetative forms in a cell. Endospores are formed by a few genera of bacteria, such as Bacillus. The Endospore stain, commonly referred to as the spore stain, is a type of staining technique. The endospore stain is a differential stain used to visualize bacterial endospores. Safranin is the secondary stain in the endospore stain and is necessary to stain the vegetative cell.Įndospore Stain. ![]()
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